Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows

Simple item page
dc.contributor.authorMüller, Heine
dc.contributor.authorHerzberg, Daniel
dc.contributor.authorChihuailaf, Ricardo
dc.contributor.authorStrobel, Pablo
dc.contributor.authorWerner, Marianne
dc.contributor.authorBustamante, Hedie
dc.date.accessioned2023-07-14T15:28:50Z
dc.date.available2023-07-14T15:28:50Z
dc.date.issued2023-05-12
dc.description.abstractInitial lameness inflammation leads to chronic lameness and development of chronic pain due to the release of pro-inflammatory mediators such as reactive oxygen species (ROS), which are implicated in the transition from acute to chronic pain, and free radical scavengers countering thiol, substance P (SP), and β-endorphin (BE). The present study aimed to evaluate the dynamic thiol–disulfide homeostasis, α-tocopherol concentrations and SP and BE concentrations in the spinal cord of chronically lame dairy cows. Ten lame and 10 non-lame cows with a parity range of 2–6 were selected for the study. Lame cows had a history of up to 3 months of lameness. Spinal cord samples were obtained from the L2 to L4 lumbar vertebrae aspect of each animal. A thiol–disulfide homeostasis assay was performed using absorbance, and the α-tocopherol concentration was determined by HPLC. SP and BE concentrations were measured using ELISA kits. The results indicated that SP and BE were significantly higher in the spinal cord of lame cows. In contrast, disulfide levels and α-tocopherol concentrations were significantly lower in the spinal cord of lame cows. In conclusion, disulfide levels and α-tocopherol concentrations indicated a defective antioxidant response in cows with chronic lameness. The results of SP and BE concentrations suggested chronic pain and a defective endogenous analgesic response.es_ES
dc.identifier.issn2076-2615
dc.identifier.other10.3390/ani13101620
dc.identifier.urihttps://hdl.handle.net/20.500.12536/1944
dc.language.isoenes_ES
dc.sourceAnimalses_ES
dc.subjectDairy cowses_ES
dc.subjectThiolses_ES
dc.subjectLamenesses_ES
dc.subjectSubstance Pes_ES
dc.subjectβ-endorphines_ES
dc.subjectα-tocopheroles_ES
dc.subjectPaines_ES
dc.titleChanges in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cowses_ES
dc.typeArtículo de revistaes_ES
uvm.escuelaEscuela de Ciencias Agrícolas y Veterinariases_ES
uvm.indexScopuses_ES
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Artículos de revista - Changes in Dynamic ThiolDisulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows.pdf
Size:
1.07 MB
Format:
Adobe Portable Document Format
Description:
Artículos de revista - Changes in Dynamic ThiolDisulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos científicos